Part:BBa_K332022:Experience

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Applications of BBa_K332022

We used RFP protein In place of the ccdB gene in our test circuit. Therefore, RFP will give us a visual approximation of how much ccdB is produced.

1. Strand D is synthesized from two fragments –

(1)Plux (2)RBS+mRFP+double ter sites

2. The plasmids are digested into corresponding products used for ligation.

3. Combine the two fragments by ligation, the protocol is the same as we have done for Strand C.

4. Transform the products from step 3 ligation.

5. Colony PCR for the transformed cells and have the PCR products electrophoresis to make sure that the fragments have been combined correctly.

6. If the electrophoresis result show that the length of Strand D is as expected, we then send the products for sequencing.

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